Yes. However they both contain the same audio data so the difference is arguably negligible. Since the audio files contain the same information such as bit rate, bit depth, dynamic range, etc. the difference comes from the way the files are read by the computer. The WAV file format is a fully uncompressed audio file. Where the FLAC / ALAC audio file is lossless compressed audio file type.

This means that when playing back the WAV audio file the computer can read the information as if you were reading a book in your native language out-loud. While playing a lossless compression file a computer program decompresses [reads] the audio file in real time for playback. This would be like trying to read the same book you were with WAV, but now the book is written in a foreign language you understand but you must translate it in your brain before reading it out-loud.

It’s true the computing program can make this translation faster than we ever could, but to perform the task it takes more processing power to read a lossless compressed audio file than a fully uncompressed audio file. This results in increased computer processing and increased power consumption, this can result in digital interference that can cause there to be some degrading of the audio quality of the lossless compressed audio file compared to a WAV file**.

**Please note that to actually hear this difference you would need to have a proper Hi-Fi audio set up.